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fluorescent probe  (MedChemExpress)
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MedChemExpress fluorescent probe
Fluorescent Probe, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fluorescent probes jc 1  (Multi Sciences (Lianke) Biotech Co Ltd)
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Multi Sciences (Lianke) Biotech Co Ltd fluorescent probes jc 1
Fluorescent Probes Jc 1, supplied by Multi Sciences (Lianke) Biotech Co Ltd, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lipid peroxidation sensitive fluorescent probe c11 bodipy  (MedChemExpress)
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MedChemExpress lipid peroxidation sensitive fluorescent probe c11 bodipy
Erastin treatment mimicked the ferroptosis phenotypes observed in cryopreserved ovarian tissue. (A) Immunohistochemical staining of GPX4 and ACSL4 in fresh ovarian tissue (Ctrl), cryopreserved tissue (Cryo), and fresh tissue treated with the ferroptosis inducer erastin (Ers). Scale bar, 50 μm. (B) Quantification of the mean optical density for GPX4 and ACSL4 staining in each group ( n = 8). (C) Measurement of Fe 2+ concentrations by colorimetric assay in the Ctrl, Cryo, and Ers groups ( n = 6). (D) Quantification of GSH levels in ovarian tissues via a colorimetric assay ( n = 5). (E) Representative confocal images of oxidized (green) and reduced <t>(red)</t> <t>C11-BODIPY</t> staining in ovarian tissues. Scale bar, 50 μm. (F) Quantification of lipid peroxidation expressed as the oxidized/reduced C11-BODIPY fluorescence ratio ( n = 5). Data are presented as mean ± SD. One-way ANOVA followed by LSD post hoc test. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
Lipid Peroxidation Sensitive Fluorescent Probe C11 Bodipy, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fluorescent probe 2  (MedChemExpress)
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MedChemExpress fluorescent probe 2
Erastin treatment mimicked the ferroptosis phenotypes observed in cryopreserved ovarian tissue. (A) Immunohistochemical staining of GPX4 and ACSL4 in fresh ovarian tissue (Ctrl), cryopreserved tissue (Cryo), and fresh tissue treated with the ferroptosis inducer erastin (Ers). Scale bar, 50 μm. (B) Quantification of the mean optical density for GPX4 and ACSL4 staining in each group ( n = 8). (C) Measurement of Fe 2+ concentrations by colorimetric assay in the Ctrl, Cryo, and Ers groups ( n = 6). (D) Quantification of GSH levels in ovarian tissues via a colorimetric assay ( n = 5). (E) Representative confocal images of oxidized (green) and reduced <t>(red)</t> <t>C11-BODIPY</t> staining in ovarian tissues. Scale bar, 50 μm. (F) Quantification of lipid peroxidation expressed as the oxidized/reduced C11-BODIPY fluorescence ratio ( n = 5). Data are presented as mean ± SD. One-way ANOVA followed by LSD post hoc test. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
Fluorescent Probe 2, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fluorescent probe trimethylammonium 1 6 diphenyl 1 3 5 hexatriene  (MedChemExpress)
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MedChemExpress fluorescent probe trimethylammonium 1 6 diphenyl 1 3 5 hexatriene
Erastin treatment mimicked the ferroptosis phenotypes observed in cryopreserved ovarian tissue. (A) Immunohistochemical staining of GPX4 and ACSL4 in fresh ovarian tissue (Ctrl), cryopreserved tissue (Cryo), and fresh tissue treated with the ferroptosis inducer erastin (Ers). Scale bar, 50 μm. (B) Quantification of the mean optical density for GPX4 and ACSL4 staining in each group ( n = 8). (C) Measurement of Fe 2+ concentrations by colorimetric assay in the Ctrl, Cryo, and Ers groups ( n = 6). (D) Quantification of GSH levels in ovarian tissues via a colorimetric assay ( n = 5). (E) Representative confocal images of oxidized (green) and reduced <t>(red)</t> <t>C11-BODIPY</t> staining in ovarian tissues. Scale bar, 50 μm. (F) Quantification of lipid peroxidation expressed as the oxidized/reduced C11-BODIPY fluorescence ratio ( n = 5). Data are presented as mean ± SD. One-way ANOVA followed by LSD post hoc test. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
Fluorescent Probe Trimethylammonium 1 6 Diphenyl 1 3 5 Hexatriene, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fluorescent probe bodipy 581 591 c11  (MedChemExpress)
96
MedChemExpress fluorescent probe bodipy 581 591 c11
Erastin treatment mimicked the ferroptosis phenotypes observed in cryopreserved ovarian tissue. (A) Immunohistochemical staining of GPX4 and ACSL4 in fresh ovarian tissue (Ctrl), cryopreserved tissue (Cryo), and fresh tissue treated with the ferroptosis inducer erastin (Ers). Scale bar, 50 μm. (B) Quantification of the mean optical density for GPX4 and ACSL4 staining in each group ( n = 8). (C) Measurement of Fe 2+ concentrations by colorimetric assay in the Ctrl, Cryo, and Ers groups ( n = 6). (D) Quantification of GSH levels in ovarian tissues via a colorimetric assay ( n = 5). (E) Representative confocal images of oxidized (green) and reduced <t>(red)</t> <t>C11-BODIPY</t> staining in ovarian tissues. Scale bar, 50 μm. (F) Quantification of lipid peroxidation expressed as the oxidized/reduced C11-BODIPY fluorescence ratio ( n = 5). Data are presented as mean ± SD. One-way ANOVA followed by LSD post hoc test. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
Fluorescent Probe Bodipy 581 591 C11, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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goat anti rabbit alexa fluorescent probe 647  (Bioss)
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Bioss goat anti rabbit alexa fluorescent probe 647
Erastin treatment mimicked the ferroptosis phenotypes observed in cryopreserved ovarian tissue. (A) Immunohistochemical staining of GPX4 and ACSL4 in fresh ovarian tissue (Ctrl), cryopreserved tissue (Cryo), and fresh tissue treated with the ferroptosis inducer erastin (Ers). Scale bar, 50 μm. (B) Quantification of the mean optical density for GPX4 and ACSL4 staining in each group ( n = 8). (C) Measurement of Fe 2+ concentrations by colorimetric assay in the Ctrl, Cryo, and Ers groups ( n = 6). (D) Quantification of GSH levels in ovarian tissues via a colorimetric assay ( n = 5). (E) Representative confocal images of oxidized (green) and reduced <t>(red)</t> <t>C11-BODIPY</t> staining in ovarian tissues. Scale bar, 50 μm. (F) Quantification of lipid peroxidation expressed as the oxidized/reduced C11-BODIPY fluorescence ratio ( n = 5). Data are presented as mean ± SD. One-way ANOVA followed by LSD post hoc test. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
Goat Anti Rabbit Alexa Fluorescent Probe 647, supplied by Bioss, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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goat anti mouse alexa fluorescent probe 488  (Bioss)
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Bioss goat anti mouse alexa fluorescent probe 488
Erastin treatment mimicked the ferroptosis phenotypes observed in cryopreserved ovarian tissue. (A) Immunohistochemical staining of GPX4 and ACSL4 in fresh ovarian tissue (Ctrl), cryopreserved tissue (Cryo), and fresh tissue treated with the ferroptosis inducer erastin (Ers). Scale bar, 50 μm. (B) Quantification of the mean optical density for GPX4 and ACSL4 staining in each group ( n = 8). (C) Measurement of Fe 2+ concentrations by colorimetric assay in the Ctrl, Cryo, and Ers groups ( n = 6). (D) Quantification of GSH levels in ovarian tissues via a colorimetric assay ( n = 5). (E) Representative confocal images of oxidized (green) and reduced <t>(red)</t> <t>C11-BODIPY</t> staining in ovarian tissues. Scale bar, 50 μm. (F) Quantification of lipid peroxidation expressed as the oxidized/reduced C11-BODIPY fluorescence ratio ( n = 5). Data are presented as mean ± SD. One-way ANOVA followed by LSD post hoc test. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
Goat Anti Mouse Alexa Fluorescent Probe 488, supplied by Bioss, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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quantagene q225 fluorescence quantitative pcr instrument  (Vazyme Biotech Co)
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Erastin treatment mimicked the ferroptosis phenotypes observed in cryopreserved ovarian tissue. (A) Immunohistochemical staining of GPX4 and ACSL4 in fresh ovarian tissue (Ctrl), cryopreserved tissue (Cryo), and fresh tissue treated with the ferroptosis inducer erastin (Ers). Scale bar, 50 μm. (B) Quantification of the mean optical density for GPX4 and ACSL4 staining in each group ( n = 8). (C) Measurement of Fe 2+ concentrations by colorimetric assay in the Ctrl, Cryo, and Ers groups ( n = 6). (D) Quantification of GSH levels in ovarian tissues via a colorimetric assay ( n = 5). (E) Representative confocal images of oxidized (green) and reduced <t>(red)</t> <t>C11-BODIPY</t> staining in ovarian tissues. Scale bar, 50 μm. (F) Quantification of lipid peroxidation expressed as the oxidized/reduced C11-BODIPY fluorescence ratio ( n = 5). Data are presented as mean ± SD. One-way ANOVA followed by LSD post hoc test. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
Quantagene Q225 Fluorescence Quantitative Pcr Instrument, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fluorescence probe jc 1  (MedChemExpress)
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MedChemExpress fluorescence probe jc 1
Erastin treatment mimicked the ferroptosis phenotypes observed in cryopreserved ovarian tissue. (A) Immunohistochemical staining of GPX4 and ACSL4 in fresh ovarian tissue (Ctrl), cryopreserved tissue (Cryo), and fresh tissue treated with the ferroptosis inducer erastin (Ers). Scale bar, 50 μm. (B) Quantification of the mean optical density for GPX4 and ACSL4 staining in each group ( n = 8). (C) Measurement of Fe 2+ concentrations by colorimetric assay in the Ctrl, Cryo, and Ers groups ( n = 6). (D) Quantification of GSH levels in ovarian tissues via a colorimetric assay ( n = 5). (E) Representative confocal images of oxidized (green) and reduced <t>(red)</t> <t>C11-BODIPY</t> staining in ovarian tissues. Scale bar, 50 μm. (F) Quantification of lipid peroxidation expressed as the oxidized/reduced C11-BODIPY fluorescence ratio ( n = 5). Data are presented as mean ± SD. One-way ANOVA followed by LSD post hoc test. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
Fluorescence Probe Jc 1, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Erastin treatment mimicked the ferroptosis phenotypes observed in cryopreserved ovarian tissue. (A) Immunohistochemical staining of GPX4 and ACSL4 in fresh ovarian tissue (Ctrl), cryopreserved tissue (Cryo), and fresh tissue treated with the ferroptosis inducer erastin (Ers). Scale bar, 50 μm. (B) Quantification of the mean optical density for GPX4 and ACSL4 staining in each group ( n = 8). (C) Measurement of Fe 2+ concentrations by colorimetric assay in the Ctrl, Cryo, and Ers groups ( n = 6). (D) Quantification of GSH levels in ovarian tissues via a colorimetric assay ( n = 5). (E) Representative confocal images of oxidized (green) and reduced (red) C11-BODIPY staining in ovarian tissues. Scale bar, 50 μm. (F) Quantification of lipid peroxidation expressed as the oxidized/reduced C11-BODIPY fluorescence ratio ( n = 5). Data are presented as mean ± SD. One-way ANOVA followed by LSD post hoc test. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Journal: Materials Today Bio

Article Title: Umbilical cord mesenchymal stem cells and their extracellular vesicles attenuate cryopreservation-induced ovarian injury via the suppression of ferroptosis in an in vitro culture system

doi: 10.1016/j.mtbio.2026.102965

Figure Lengend Snippet: Erastin treatment mimicked the ferroptosis phenotypes observed in cryopreserved ovarian tissue. (A) Immunohistochemical staining of GPX4 and ACSL4 in fresh ovarian tissue (Ctrl), cryopreserved tissue (Cryo), and fresh tissue treated with the ferroptosis inducer erastin (Ers). Scale bar, 50 μm. (B) Quantification of the mean optical density for GPX4 and ACSL4 staining in each group ( n = 8). (C) Measurement of Fe 2+ concentrations by colorimetric assay in the Ctrl, Cryo, and Ers groups ( n = 6). (D) Quantification of GSH levels in ovarian tissues via a colorimetric assay ( n = 5). (E) Representative confocal images of oxidized (green) and reduced (red) C11-BODIPY staining in ovarian tissues. Scale bar, 50 μm. (F) Quantification of lipid peroxidation expressed as the oxidized/reduced C11-BODIPY fluorescence ratio ( n = 5). Data are presented as mean ± SD. One-way ANOVA followed by LSD post hoc test. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Lipid peroxidation in ovarian tissues was assessed using the lipid peroxidation sensitive fluorescent probe C11-BODIPY (581/591) (MedChemExpress, USA), which shifts from red to green fluorescence upon oxidation.

Techniques: Immunohistochemical staining, Staining, Colorimetric Assay, Fluorescence

UC-MSCs alleviated cryopreservation-induced ovarian injury by inhibiting ferroptosis. (A) Immunohistochemical staining of GPX4 and ACSL4 in the four experimental groups. Scale bar, 50 μm. (B) Quantification of the mean optical density for GPX4 and ACSL4 staining ( n = 8). (C) Measurement of the Fe 2+ concentration in ovarian tissues via a colorimetric assay ( n = 6). (D) Quantification of GSH levels via colorimetric assay ( n = 5). (E) CD31 immunohistochemical staining was used to assess microvessel density in ovarian tissue. Scale bar, 50 μm. (F) Quantitative analysis of CD31-positive microvessel density ( n = 5). (G) RT-qPCR analysis of the expression of angiogenesis-related genes, including VEGF, ANG2, and IGF1 ( n = 5). (H) Representative confocal images of oxidized (green) and reduced (red) C11-BODIPY. Scale bar, 50 μm. (I) Quantification of the oxidized/reduced C11-BODIPY fluorescence ratio ( n = 3). Data are presented as mean ± SD. Student's t -test or one-way ANOVA with LSD post hoc tests was used. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Journal: Materials Today Bio

Article Title: Umbilical cord mesenchymal stem cells and their extracellular vesicles attenuate cryopreservation-induced ovarian injury via the suppression of ferroptosis in an in vitro culture system

doi: 10.1016/j.mtbio.2026.102965

Figure Lengend Snippet: UC-MSCs alleviated cryopreservation-induced ovarian injury by inhibiting ferroptosis. (A) Immunohistochemical staining of GPX4 and ACSL4 in the four experimental groups. Scale bar, 50 μm. (B) Quantification of the mean optical density for GPX4 and ACSL4 staining ( n = 8). (C) Measurement of the Fe 2+ concentration in ovarian tissues via a colorimetric assay ( n = 6). (D) Quantification of GSH levels via colorimetric assay ( n = 5). (E) CD31 immunohistochemical staining was used to assess microvessel density in ovarian tissue. Scale bar, 50 μm. (F) Quantitative analysis of CD31-positive microvessel density ( n = 5). (G) RT-qPCR analysis of the expression of angiogenesis-related genes, including VEGF, ANG2, and IGF1 ( n = 5). (H) Representative confocal images of oxidized (green) and reduced (red) C11-BODIPY. Scale bar, 50 μm. (I) Quantification of the oxidized/reduced C11-BODIPY fluorescence ratio ( n = 3). Data are presented as mean ± SD. Student's t -test or one-way ANOVA with LSD post hoc tests was used. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Lipid peroxidation in ovarian tissues was assessed using the lipid peroxidation sensitive fluorescent probe C11-BODIPY (581/591) (MedChemExpress, USA), which shifts from red to green fluorescence upon oxidation.

Techniques: Immunohistochemical staining, Staining, Concentration Assay, Colorimetric Assay, Quantitative RT-PCR, Expressing, Fluorescence

MSC-EVs repaired cryopreservation-induced ovarian injury by inhibiting ferroptosis. (A) Immunohistochemical staining of GPX4 and ACSL4 in the four experimental groups. Scale bar, 50 μm. (B) Quantification of the mean optical density for GPX4 and ACSL4 staining ( n = 5). (C) Quantification of Fe 2+ concentrations in ovarian tissue via a colorimetric assay ( n = 5). (D) Quantification of GSH levels via colorimetric assay ( n = 5). (E) Representative confocal images of oxidized (green) and reduced (red) C11-BODIPY. Scale bar, 50 μm. (F) Quantification of the oxidized/reduced C11-BODIPY fluorescence ratio ( n = 3). Data are presented as mean ± SD. One-way ANOVA followed by LSD post hoc test. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Journal: Materials Today Bio

Article Title: Umbilical cord mesenchymal stem cells and their extracellular vesicles attenuate cryopreservation-induced ovarian injury via the suppression of ferroptosis in an in vitro culture system

doi: 10.1016/j.mtbio.2026.102965

Figure Lengend Snippet: MSC-EVs repaired cryopreservation-induced ovarian injury by inhibiting ferroptosis. (A) Immunohistochemical staining of GPX4 and ACSL4 in the four experimental groups. Scale bar, 50 μm. (B) Quantification of the mean optical density for GPX4 and ACSL4 staining ( n = 5). (C) Quantification of Fe 2+ concentrations in ovarian tissue via a colorimetric assay ( n = 5). (D) Quantification of GSH levels via colorimetric assay ( n = 5). (E) Representative confocal images of oxidized (green) and reduced (red) C11-BODIPY. Scale bar, 50 μm. (F) Quantification of the oxidized/reduced C11-BODIPY fluorescence ratio ( n = 3). Data are presented as mean ± SD. One-way ANOVA followed by LSD post hoc test. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Lipid peroxidation in ovarian tissues was assessed using the lipid peroxidation sensitive fluorescent probe C11-BODIPY (581/591) (MedChemExpress, USA), which shifts from red to green fluorescence upon oxidation.

Techniques: Immunohistochemical staining, Staining, Colorimetric Assay, Fluorescence